Hcs cellmask
WebHCS CellMask™ stains label the entire cell and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in … WebIdentify entire cells (cytoplasm and nucleus) during high-content screening (HCS) assays with the versatile HCS CellMask Red, CellMask Orange, …
Hcs cellmask
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WebThe versatile HCS CellMask Red, CellMask Orange, CellMask Green, CellMask Blue, CellMask Deep Red, and CellMask Near-IR stains are cell delineation tools for high … WebHCS CellMask stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell … HCS CellMask stains can be applied to cells immediately after fixation or in the last … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
WebA multiplexed fluorescent dye set labeling the SARS-CoV-2 nucleocapsid protein (N), nuclei (Hoechst 33342), neutral lipids (HCS LipidTox Green), and cytoplasm (HCS CellMask Orange) was used to capture a wide … WebHCS NuclearMask ™ stains) or the entire cell (Invitrogen ™ HCS CellMask ™ stains). Once the nucleus or cell is recognized as an object to analyze, the software can quantify additional fluorescent reporters for various cellular processes. Figure 1. Cytochalasin D disrupts actin filaments and reduces the total area of actin in cells. (A)
WebApr 13, 2024 · To stain cells with HCS CellMask, in each well, 2 mL Triton X-100 PBS solution (5,000-fold dilution) was applied and incubate for 15 min; cells were washed twice to remove Triton X-100; 2 mL HCS CellMask solution (working conc.: 0.5 μg/mL in PBS buffer) was added and incubated for 30 min; cells were washed 2–3 times with PBS buffer. ... WebHCS CellMask stains are available in a range of fluorescent colors, they can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are …
WebMar 9, 2015 · CellMask™ Plasma Membrane Stains 4 (Optional) Fixation after staining 2.1 Remove the staining solution and fix the cells after staining (step 1.3) with warm 3.75% …
WebJan 19, 2024 · Spheroids were also stained with DAPI to detect nuclei and with CellMask to demarcate the outer, peripheral edges of the spheroid. (a) Imaging of the whole spheroid. Confocal image stacks through the body of the spheroid were acquired on a Leica SP8 confocal microscope. Images shown are maximum intensity z-projections. Scale bar 20 μm. 2 x 計算WebHCS CellMask™ stains label the entire cell and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. 2 x 導関数WebAug 13, 2016 · HCS CellMask ™ Stains are available in either blue, green, orange, red, or deep red detection spectra. We used HCS CellMask™ R ed stain to label the whole cell … 2 x泰勒公式WebHCS CellMask™ stains are applied to cells immediately after fixation and permeabilization or after antibody labeling. Sufficient quantities are provided to stain ten 96-well plates … 2 x 定積分Web1. ™Prepare a 10-mg/mL HCS CellMask stock solution by dissolving the entire contents of Component A in 25 μL of Component B. 2. Prepare 4% paraformaldehyde and 0.1% Triton® X-100 solutions (available separately in the Image-iT® Fixation/Permeabilization Kit, Cat. no. R37602). 3. ™Prepare HCS CellMask staining solution by adding 2 μL of HCS 2 上海乾铄精密塑胶有限公司WebInvitrogen™ HCS CellMask™ Stains Easily visualize entire cells or individual cell structures during high-content screening (HCS) assays with HCS CellMask stains, which are … 2 x n 타일링WebMay 11, 2024 · HCS CellMask™ Green Stain: ThermoFisher Scientific: Cat# H32714: HCS CellMask™ Deep Red Stain: ThermoFisher Scientific: Cat# H32721: Hoechst 33258: Sigma-Aldrich: Cat# 861405: RPMI 1640 with 1x L-Glutamine: ... Microscopy data were analyzed using HCS Studio quantitative analysis software. Immunoblots were analyzed … 2 xy平面内一薄板 在x方向上受均匀拉力q作用 y方向受均匀压力p作用